Plasmid

Part:BBa_K4823007:Design

Designed by: Zike Yao   Group: iGEM23_HKSSC   (2023-10-10)


pET_21a (+) _qSCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a lac operon within the plasmid backbone, to prevent leaky expression of protein, we deleted the lac operon before proceeding to expression.


Source

Pseudomonas aeruginosa PAO1

References